Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

11.9K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
11.9K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

2.5K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
2.5K
Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
Ligand Binding Sites02:40

Ligand Binding Sites

12.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Choline supplementation partially reverses prefrontal neurochemical deficits induced by perinatal opioid exposure.

Scientific reports·2026
Same author

Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.

ACS chemical neuroscience·2026
Same author

A brain-gut excitatory peptide/CCHamide homolog regulates satiation and motivational state transitions in the Aplysia feeding circuit.

The Journal of biological chemistry·2026
Same author

Metabolite profiling of the effect of prenatal stimuli across postnatal treatments in the liver.

Molecular and cellular endocrinology·2026
Same author

Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum.

Analytical chemistry·2025
Same author

Metabolic and Inflammatory Stimuli Impact Vascular Circulation and Cell Proliferation Processes in the Amygdala.

Genes, brain, and behavior·2025

Related Experiment Video

Updated: Jun 29, 2025

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

2.4K

Bioinformatics for Prohormone and Neuropeptide Discovery.

Bruce R Southey1, Elena V Romanova2, Sandra L Rodriguez-Zas1

  • 1Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2024
PubMed
Summary

We developed a method to identify prohormones and neuropeptides from genomic data. This approach aids in annotating genomes and discovering new signaling molecules for physiological and behavioral research.

Keywords:
BioinformaticsCleavageGene predictionHomologyNeuropeptideProhormone

More Related Videos

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K
MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease
16:57

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

Published on: February 14, 2012

26.4K

Related Experiment Videos

Last Updated: Jun 29, 2025

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

2.4K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K
MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease
16:57

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

Published on: February 14, 2012

26.4K

Area of Science:

  • Biochemistry
  • Genomics
  • Neuroscience

Background:

  • Neuropeptides and peptide hormones are crucial signaling molecules derived from prohormones through posttranslational modifications.
  • These molecules regulate diverse physiological systems and behaviors by activating specific receptors.

Purpose of the Study:

  • To present a validated methodology for identifying prohormone complements within genomic assemblies.
  • To facilitate the discovery and annotation of neuropeptides for proteomic research.

Main Methods:

  • Utilized publicly available toolsets and databases for prohormone identification.
  • Developed a screening process for putative neuropeptides from identified prohormone sequences.

Main Results:

  • Successfully identified prohormone sequences from genomic assemblies.
  • Established a pipeline for subsequent neuropeptide discovery and validation.

Conclusions:

  • The described methodology provides a robust framework for genomic annotation of neuropeptide systems.
  • Enables accurate proteomic discovery and validation of neuropeptides and their precursors.