Related Experiment Video
Updated: Jun 29, 2025
09:41
An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
Published on: July 15, 2015
8.7K
Low TYROBP expression predicts poor prognosis in multiple myeloma.
Hong Luo1,2, Chengyun Pan1, Li Wang1,2
1Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China.
Cancer Cell International
|March 29, 2024
Summary
TYROBP is a potential prognostic marker for multiple myeloma (MM), a refractory hematologic cancer. Down-regulation of TYROBP indicates a poor prognosis and affects MM development via cell adhesion pathways.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Multiple myeloma (MM) is a significant refractory hematologic cancer.
- Identifying novel prognostic markers is crucial for MM management.
Purpose of the Study:
- To identify novel prognostic markers for multiple myeloma (MM).
- To investigate the role of TYROBP in MM development and progression.
Main Methods:
- Differential gene expression analysis and Weighted Gene Co-expression Network Analysis (WGCNA) were used to identify MM-related genes.
- Protein-protein interaction (PPI) analysis identified hub genes, including TYROBP.
- Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were performed for functional and pathway analysis.
- Western blotting, RT-PCR, cell adhesion, and transwell assays validated TYROBP's role.
Main Results:
- 92 differentially expressed genes (DEGs) and 10 hub genes were identified.
- Down-regulation of TYROBP correlated with dismal MM prognosis.
- TYROBP independently predicted MM prognosis and was linked to the cell adhesion pathway.
- TYROBP expression was decreased in MM patients, and its up-regulation increased MM cell adhesion and decreased migration.
Conclusions:
- TYROBP is a potential prognostic marker for multiple myeloma (MM).
- TYROBP plays a role in MM cell adhesion and migration, influencing disease progression.
Related Concept Videos
01:15Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K