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Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
Published on: May 12, 2020
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Microbial Spectrum of Keratitis at a Rural Tertiary Care Teaching Hospital.
Bipin Chandra Bhagath1, Subbarama Prasad1, Arvind Natarajan1
1Microbiology, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, IND.
Cureus
|March 29, 2024
Summary
Microbial keratitis is often caused by fungi like Fusarium and bacteria such as Streptococcus pneumoniae, particularly affecting farmers. Understanding these microbial profiles is key for effective treatment.
Area of Science:
- Ophthalmology
- Microbiology
- Infectious Diseases
Background:
- Microbial keratitis is a significant cause of vision impairment.
- Causative agents include bacteria, fungi, viruses, and parasites.
- Microbial profiles vary by demographics, location, climate, and occupation.
Purpose of the Study:
- To investigate the microbiological profile of microbial keratitis.
- To identify common causative agents and risk factors.
- To inform treatment strategies.
Main Methods:
- Collected corneal scrapings from 75 patients diagnosed with microbial keratitis.
- Processed samples in a microbiology laboratory.
- Identified bacterial and fungal isolates using standard procedures.
Main Results:
- Fungi (68.42%) were more prevalent than bacteria (31.57%) in identified cases.
- Fusarium (53.84%) and Aspergillus (23.07%) were the most common fungi.
- Streptococcus pneumoniae (83.33%) was the primary bacterial isolate.
- Farmers (66.67%) and individuals aged 21-60 were most affected.
- Higher incidence observed from November to February.
Conclusions:
- Regularly updating local microbial profiles is crucial.
- Understanding antimicrobial resistance patterns aids treatment decisions.
- Informed selection of topical treatments improves patient outcomes.
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