miR-200c-141 induces a hybrid E/M state and promotes collective cell migration in MDA-MB-231 cells
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View abstract on PubMed
Summary
This summary is machine-generated.The microRNA-200 (miR-200) family can induce a hybrid epithelial/mesenchymal (E/M) state in cancer cells. This hybrid state promotes collective cell migration, contributing to cancer malignancy.
Area Of Science
- Molecular Biology
- Cancer Research
- Cell Biology
Background
- The microRNA-200 (miR-200) family is known to suppress epithelial-to-mesenchymal transition (EMT).
- Recent studies indicate miR-200 can paradoxically promote cancer progression in certain contexts.
- The hybrid epithelial/mesenchymal (E/M) state is linked to increased cancer malignancy.
Purpose Of The Study
- To investigate the role of the miR-200 family in inducing a hybrid E/M state.
- To examine the effect of miR-200 family members on mesenchymal MDA-MB-231 cells.
- To understand the contribution of miR-200 to cancer malignancy.
Main Methods
- Induction of miR-200c-141 cluster in mesenchymal cells.
- Analysis of epithelial and mesenchymal gene expression.
- Assessment of cell-cell junction formation and cell migration.
Main Results
- Induction of miR-200c-141 led to epithelial gene expression and cell-cell junction formation.
- Mesenchymal markers were retained in cells induced with miR-200c-141.
- miR-200c-141 induction promoted collective migration with F-actin cable formation.
Conclusions
- The miR-200 family can induce a hybrid E/M state in mesenchymal cancer cells.
- This hybrid E/M state is associated with enhanced collective cell migration.
- The miR-200 family's role in cancer progression is complex and context-dependent.
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