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Related Experiment Video

Updated: Jun 29, 2025

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Published on: June 12, 2015

Formation of vascular-like structures using a chemotaxis-driven multiphase model.

Georgina Al-Badri¹, James B Phillips², Rebecca J Shipley³

¹Department of Mathematics, University College London, London, UK; Centre for Nerve Engineering, University College London, London, UK.

Mathematical Biosciences

|March 30, 2024

Summary

This study introduces a multiphase model for cell patterning in extracellular matrix (ECM). The model simulates chemotaxis-driven cell migration, forming vascular-like structures and identifying key parameters for in vitro vascular network formation.

Keywords:

Chemotaxis Multiphase modelling Tissue engineering Vasculogenesis

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Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

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Last Updated: Jun 29, 2025

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

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Published on: June 12, 2015

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

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Published on: October 13, 2019

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

Area of Science:

Computational Biology
Biophysics
Tissue Engineering

Background:

Cell patterning is crucial for tissue development and function.
Understanding in vitro cell organization is key for tissue engineering applications.

Purpose of the Study:

To develop and validate a continuum multiphase model for simulating in vitro cell patterning.
To investigate mechanisms driving pattern formation, including chemotaxis and cell-matrix interactions.
To model the formation of vascular-like structures in 1D and 2D.

Main Methods:

Utilized a multiphase model framework for continuum cell patterning.
Incorporated chemotaxis-driven cell migration, cell-matrix traction, contact inhibition, and cell-cell aggregation.
Performed sensitivity analysis to identify critical model parameters.
Included chemoattractant-matrix binding to refine spatial scales.

Main Results:

Chemotaxis-driven migration successfully generated cell clusters (1D) and vascular-like structures (2D).
Cell-matrix traction, contact inhibition, and aggregation influenced pattern formation.
Chemoattractant-matrix binding reduced patterning scale to biologically relevant ranges.
1D model findings translated effectively to 2D simulations, yielding vascular patterns.

Conclusions:

The developed multiphase model accurately simulates long-term in vitro cell pattern formation.
The model provides a biologically plausible framework for studying vascular network development.
Key parameters influencing pattern formation were identified, aiding future experimental design.