Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Newton-X platform for mixed quantum-classical dynamics.

Physical chemistry chemical physics : PCCP·2026

Same author

IL-9 orchestrates MDSC expansion and inflammatory programming to amplify immunopathology during experimental cerebral malaria.

Microbial pathogenesis·2026

Same author

Unveiling the Potential Therapeutic Efficacy of Gold Ceria Nanohybrid as an Anticancer Agent: A Special Insight to Molecular Docking Study.

ACS applied bio materials·2026

Same author

Enhanced Taylor-Aris dispersion in slender pulsatile annular channels: Implications for perivascular solute transport.

Journal of fluid mechanics·2026

Same author

Understanding Size Distributions during Lipid Nanoparticle Manufacturing through Mechanistic Modeling.

ACS omega·2026

Same author

Secure implantable cardiac pacemaker for medical consumer electronics.

npj biomedical innovations·2026

Search research articles

Related Experiment Video

Updated: May 5, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Estimating nitric oxide (NO) from MDSCs by Griess method.

Pronabesh Ghosh¹, Saikat Mukherjee¹, Soubhik Ghosh¹

¹Immunology Laboratory, Department of Zoology, University of Calcutta, Kolkata, India.

Methods in Cell Biology

|March 30, 2024

Summary

Nitric oxide (NO) plays key roles in immunity. This study details a method to measure NO secretion from myeloid-derived suppressor cells (MDSCs) using the Griess assay.

Keywords:

Griess method MDSC NOS Nitric oxide

More Related Videos

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Published on: April 21, 2023

Related Experiment Videos

Last Updated: May 5, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Published on: April 21, 2023

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Nitric oxide (NO) is crucial in immunology, mediating antimicrobial, anti-tumoral, anti-inflammatory, and immunosuppressive functions.
Nitrogen oxide synthase (NOS) synthesizes NO, with isoforms acting in a cell-dependent manner. NO rapidly oxidizes to Reactive Nitrogen Oxide Species (RNOS).
Myeloid-derived suppressor cells (MDSCs) are significant NO secretors, influencing T-cell proliferation, differentiation, and natural killer (NK) cell functions.

Purpose of the Study:

To detail a method for estimating nitric oxide (NO) secretion from myeloid-derived suppressor cells (MDSCs).
To provide a reliable protocol for quantifying NO production, essential for understanding its immunological roles.

Main Methods:

Utilizes the Griess reagent assay, a convenient colorimetric method for NO quantification.
Involves the reaction of NO with Griess reagents to form a detectable azo dye.
Detection of the azo dye is performed using a microplate reader at a wavelength of 548nm.

Main Results:

The Griess method provides a quantitative measure of NO secreted by MDSCs.
This method allows for the assessment of NO's contribution to MDSC-mediated immune modulation.
The protocol is optimized for microplate-based detection.

Conclusions:

The Griess assay is an effective and convenient method for measuring NO secretion from MDSCs.
Accurate NO quantification is vital for studying its complex roles in immune suppression and other immunological processes.
This detailed method facilitates further research into NO's functional importance in immunology.