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Updated: Jun 29, 2025
11:02
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
527
[Standardization and Practical Application of Quantitative NMR (qNMR)]
1National Institute of Health Sciences.
Summary
Quantitative NMR (qNMR) is now internationally standardized for purity determination, enabling new analytical methods. This advancement facilitates accurate compound quantification in food and pharmaceutical products.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Organic Chemistry
Context:
- Quantitative NMR (qNMR) is established in Japan for sample purity determination.
- International standardization was initiated due to the lack of a global consensus on qNMR methods.
- Japan proposed the initiative for international standardization of qNMR.
Purpose:
- To establish international requirements for purity determination using qNMR.
- To publish ISO 24583:2022, outlining general requirements for the 1H-NMR internal standard method.
- To facilitate global adoption of standardized qNMR techniques.
Summary:
- ISO 24583:2022 standardizes quantitative nuclear magnetic resonance spectroscopy for purity determination of organic compounds in food.
- A novel chromatographic quantification method using relative molar sensitivity (RMS) determined by qNMR is introduced.
- This method combines qNMR and chromatography for accurate analyte quantification without needing analyte reference material.
Impact:
- Provides an internationally agreed-upon set of requirements for qNMR-based purity determination.
- Enables new analytical technologies, such as chromatographic quantification using RMS.
- Facilitates accurate content determination of compounds in food and pharmaceutical products, with adoption in Japanese Pharmacopoeia and consideration for JAS.
Keywords:
practical applicationquantitative NMR (qNMR)relative molar sensitivity (RMS)standardizationMore Related Videos
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