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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy
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RNA binding proteins in cardiovascular development and disease.

Sunil K Verma1, Muge N Kuyumcu-Martinez2

  • 1Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine Charlottesville, VA, United States.

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|March 31, 2024
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Mutations in RNA binding proteins (RBPs) contribute to congenital heart disease (CHD), the most common birth defect. Understanding RBPs in heart development may lead to novel RNA-based treatments for CHD.

Keywords:
Alternative polyadenylationAlternative splicingCongenital heart defectsHeart developmentRNA binding proteinmRNA

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Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Developmental Biology

Background:

  • Congenital heart disease (CHD) is a prevalent birth defect with significant mortality and lifelong complications.
  • Mutations or variants in RNA binding proteins (RBPs) are increasingly recognized as contributing factors to CHD.
  • The specific roles of RBPs in embryonic heart development and their direct links to CHD remain largely unexplored.

Purpose of the Study:

  • To review current knowledge on specific RBPs implicated in congenital heart disease.
  • To highlight the significance of RBPs as regulators of gene expression in cardiac development.
  • To underscore the potential of RNA-based therapies for treating CHD.

Main Methods:

  • Literature review of studies investigating RBPs in congenital heart disease.
  • Analysis of the known functions of RBPs in gene regulation, particularly mRNA processing and protein production.
  • Exploration of emerging RNA-based methodologies and therapies applicable to CHD research.

Main Results:

  • RBPs are crucial regulators of the mRNA and protein landscape, influencing cellular function.
  • Specific RBPs have been identified as potential contributors to the pathogenesis of CHD.
  • The developing heart's reliance on precise gene regulation makes it vulnerable to RBP dysfunction.

Conclusions:

  • Further investigation into RBPs' roles in heart development is essential for understanding CHD.
  • Identifying RBP target RNAs in the embryonic heart can elucidate disease mechanisms.
  • This research paves the way for developing innovative RNA-based therapeutic strategies for congenital heart disease.