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Updated: Jun 29, 2025

On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

CD151 Maintains Endolysosomal Protein Quality to Inhibit Vascular Inflammation.

Junxiong Chen¹, Yingjun Ding¹, Chao Jiang¹

¹University of Oklahoma Health Sciences Center, Oklahoma City, OK (J.C., Y.D., C.J., R.Q., X.W., D.N.R., B.L., J.D., B.S., J.W., H.Z., C.H., K.-M.F., H.C.R., M.S.W., T.K., F.A.H., J.F.P., X.A.Z.).

Circulation Research

|April 1, 2024

Summary

Tetraspanin CD151, crucial for endothelial cell adhesion, plays a vital role in preventing vascular inflammation by maintaining endolysosome function and protein quality control. Its downregulation exacerbates inflammation, highlighting its therapeutic potential.

Keywords:

cell adhesion endothelial cells multivesicular bodies proteolysis ubiquitin

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Area of Science:

Vascular Biology
Cellular Biology
Immunology

Background:

Tetraspanin CD151 is highly expressed in endothelial cells, reinforcing cell adhesion.
The specific role of CD151 in vascular inflammation is not well understood.

Purpose of the Study:

To investigate the role of CD151 in endothelial cells during vascular inflammation.
To elucidate the molecular mechanisms by which CD151 regulates inflammation.

Main Methods:

In vitro studies using genetically modified endothelial cells.
In vivo studies using genetically engineered mouse models.
In silico systems biology and bioinformatics analyses.

Main Results:

Endothelial CD151 ablation leads to pulmonary and cardiac inflammation, sepsis, and exacerbates COVID-19.
CD151 restrains endothelial release of proinflammatory molecules, reducing leukocyte infiltration.
CD151 maintains endolysosome integrity, controlling exosome production and protein turnover via VCP/p97 and IFITM3.

Conclusions:

CD151 maintains endolysosome function and protein quality control, limiting vascular inflammation.
The IFITM3/V-ATPase-tetraspanin-VCP/p97 complex acts as a protein quality control and anti-inflammatory machinery.
Targeting this complex may offer therapeutic benefits for vascular inflammation.