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Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research
C D Corcini1, J Vilela2, N Gatti3
1Center for Research and Education in Animal Reproduction, Federal University of Pelotas, Faculdade de Veterinaria, Campus Universitario, S/N - CEP 96160-000, Capao do Leao, RS - Brasil. corcinicd@gmail.com.
Background:
Examining semen cryopreservation in Calomys laucha offers valuable insights for reproductive research and species conservation.
Objective:
To determine the most effective sugar for the cryopreservation of C. laucha semen.
Materials And Methods:
Using 36 epididymides from C. laucha, semen samples were diluted in a 3% skimmed milk medium supplemented with one of four sugars (glucose, fructose, lactose, or sucrose) at a concentration of 0.3 M. These mixtures underwent a conditioning phase at 37 degree C for 10 min, cooled to -80 degree C for another 10 min, and were subsequently stored in liquid nitrogen.
Results:
Upon thawing, samples treated with lactose and glucose solutions show superior sperm motility, achieving 8.2% and 10.0% respectively, in contrast to the fructose (2.0%) and sucrose (4.1%) mixtures. Furthermore, samples preserved in glucose registered the highest sperm penetration rates, reaching 44.9%.
Conclusion:
Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen. https://doi.org/10.54680/fr24210110612.
