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Protein Dynamics in Living Cells

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Updated: Jun 29, 2025

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Assessing Aptamer-Analyte Binding Kinetics by Microfluidic Fluorescence Microscopy.

Kechun Wen¹, Xin Meng¹, Chengxi Wang¹

¹Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA.

Sensors and Actuators. B, Chemical

|April 1, 2024

Summary

This summary is machine-generated.

This study introduces a cost-effective fluorescence microscopy assay for aptamer-binding kinetics. The method uses microfluidics for accessible, rapid analysis in resource-limited labs.

Keywords:

aptamer fluorescence microscopy kinetics microfluidics

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Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Kinetic measurement is vital for aptamer selection and understanding binding mechanisms.
Existing methods are often costly, require large sample volumes, and specialized equipment, limiting accessibility.

Purpose of the Study:

To develop a simple, cost-effective fluorescence microscopy-based microfluidic assay for measuring aptamer-analyte binding kinetics.
To provide an accessible alternative for aptamer kinetic analysis, particularly for resource-limited laboratories.

Main Methods:

A microfluidic chip is used to immobilize targets (proteins or cells).
Fluorescently labeled aptamers are introduced, and their binding kinetics are monitored over time using standard fluorescence microscopy.
The assay leverages microfluidic flow for efficient aptamer-target interaction and measurement.

Main Results:

The assay successfully measured aptamer binding kinetics to immunoglobulin E protein and CCRF-CEM cells.
Results obtained were consistent with established, more complex methods.
Demonstrated the assay's utility for exploring aptamer-target interactions.

Conclusions:

The developed fluorescence microscopy-based microfluidic assay offers a simple, cost-effective, and accessible method for aptamer-binding kinetic measurements.
This technique can aid in identifying optimal aptamers for diverse biomedical applications.
Enhances the capabilities of research laboratories with modest resources for aptamer research.