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Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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A Single Electrochemical Biosensor Designed to Detect Any Virus.

Fiorella Torres-Salvador1, Julio Ojeda1, Cynthia Castro1

  • 1Department of Chemistry, University of Central Florida, Orlando, Florida 32816, United States.

Analytical Chemistry
|April 1, 2024
PubMed
Summary
This summary is machine-generated.

This study presents a novel electrochemical biosensor (E-Biosensor) for virus detection. By modifying Nucleic Acid Sequence-Based Amplification (NASBA), this method enables sensitive detection of multiple viruses using a single biosensor design.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Technology

Background:

  • Viruses cause significant human and animal diseases.
  • Viral detection often requires RNA amplification, with RT-qPCR and NASBA being common methods.
  • Nucleic Acid Sequence-Based Amplification (NASBA) offers isothermal amplification and high selectivity, suitable for resource-limited settings.

Purpose of the Study:

  • To develop a versatile virus detection platform using a single electrochemical biosensor (E-Biosensor).
  • To adapt the Nucleic Acid Sequence-Based Amplification (NASBA) protocol for integration with the E-Biosensor.
  • To demonstrate the E-Biosensor's capability in detecting multiple distinct viruses.

Main Methods:

  • A modified NASBA protocol was developed using a reverse primer with an additional 5' tag region.
  • The tag region on the NASBA amplicon facilitates hybridization with specific E-Biosensor probes.
  • A single E-Biosensor design was utilized for detecting multiple viruses.

Main Results:

  • The modified NASBA protocol successfully generated amplicons containing the tag region.
  • The E-Biosensor effectively detected the presence of three different viruses.
  • The single E-Biosensor design demonstrated versatility across multiple viral targets.

Conclusions:

  • The developed E-Biosensor platform, coupled with modified NASBA, offers a promising approach for broad-spectrum virus detection.
  • This method is adaptable for detecting various viruses using a single, standardized biosensor.
  • The technology holds potential for applications in diverse diagnostic settings, including low-resource environments.