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Related Experiment Video

Updated: Jun 29, 2025

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
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Thermal-tagging photoacoustic remote sensing flowmetry.

Yun Lu, Yunxu Sun, Zhousheng Shen

    Optics Letters
    |April 1, 2024
    PubMed
    Summary

    Photoacoustic remote sensing (PARS) overcomes ultrasound coupling challenges in microscopy. A new thermal-tagging flowmetry method enables non-contact, dye-free blood flow imaging with high accuracy.

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    Area of Science:

    • Biomedical Optics
    • Medical Imaging
    • Flowmetry

    Background:

    • Traditional photoacoustic microscopy (PAM) faces ultrasound coupling challenges for clinical applications like chronic wound imaging.
    • Photoacoustic remote sensing (PARS) offers an all-optical alternative, avoiding ultrasound coupling limitations.
    • While PARS excels in histopathology and oxygen metabolism imaging, its hemodynamic quantification capabilities are underexplored.

    Purpose of the Study:

    • To introduce and validate an all-optical thermal-tagging flowmetry approach for PARS microscopy.
    • To assess the performance of this method for hemodynamic quantification in a non-contact, dye-free manner.
    • To address the unexplored potential of PARS in blood flow imaging.

    Main Methods:

    • Development of an all-optical thermal-tagging flowmetry technique integrated with PARS microscopy.

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  • Comprehensive mathematical modeling to underpin the flowmetry approach.
  • Ex vivo and in vivo experimental validation of the developed method.

    • Main Results:

    • The thermal-tagging flowmetry demonstrated a detectable blood flow rate range of 0 to 12 mm/s.
    • High measurement accuracy was achieved, with a reported error of ±1.2%.
    • Successful operation was confirmed at a 10-kHz laser pulse repetition rate.

    Conclusions:

    • The proposed all-optical thermal-tagging flowmetry is an effective method for PARS microscopy.
    • This technique enables non-contact, dye-free hemodynamic imaging.
    • It presents a significant advancement for PARS applications in clinical settings and research.