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Updated: May 6, 2026

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

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Widefield functional speckle-correlation optical scattering mesoscopy toward hemodynamic imaging.

Ruifeng Zhang, Liang Fei, Xiangru Liu

|April 1, 2024

Summary

This study introduces a widefield functional SCOSI (WF-SCOSI) system for non-invasive biomedical imaging. The new system overcomes field-of-view limitations, enabling detailed mapping of fluid dynamics in biological tissues.

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Last Updated: May 6, 2026

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Published on: October 2, 2021

Area of Science:

Biomedical Optics
Medical Imaging
Fluid Dynamics

Background:

Speckle-correlation optical scattering imaging (SCOSI) offers non-invasive deep tissue imaging by analyzing scattering patterns.
Limitations in SCOSI include a restricted field of view due to the optical memory effect and underdeveloped functional imaging capabilities.
Preclinical translation of SCOSI for biomedical applications is hindered by these challenges.

Purpose of the Study:

To present a proof-of-concept for the first widefield functional SCOSI (WF-SCOSI) system.
To enable simultaneous mesoscopic mapping of fluid morphology and flow rate.
To advance non-invasive functional imaging in biological tissues.

Main Methods:

Implementation of scanning synthesis techniques.
Development of fluorescence scattering flowmetry.
Design and testing of a first-generation WF-SCOSI system.

Main Results:

Demonstrated simultaneous mesoscopic mapping of fluid morphology and flow rate.
Successfully imaged fluorescence-labeled blood vessel networks ex vivo.
Validated the effectiveness of WF-SCOSI under strong scattering conditions.

Conclusions:

The WF-SCOSI system overcomes previous field-of-view limitations.
This technology is effective for non-invasive hemodynamic imaging.
WF-SCOSI shows significant potential for preclinical biomedical diagnostic applications.