Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame Topic

Quantifying optical sectioning in reflection microscopy with patterned illumination.

Biomedical optics express·2026

Same author

CbuBIM1 Antagonizes CbuMYB27 to Regulate Tension Wood Formation in Catalpa bungei.

Plant, cell & environment·2026

Same author

Integrated Reanalysis of Global Riverine Fish eDNA Datasets Shows Robustness and Congruence of Biodiversity Conclusions.

Molecular ecology·2026

Same author

Deep transfer learning radiomics combined with explainable machine learning for predicting malignancy risk in parotid gland tumors based on ultrasound.

European journal of radiology·2026

Same author

Rhein/Fe<sup>3+</sup> nanoassembly for triple-negative breast cancer treatment via a ferroptosis and immunogenic death.

Chinese herbal medicines·2026

Same author

AdaEndoGS: An Adaptive Enlightening Model for Endoscopy Based on 3D Gaussian Splatting.

IEEE transactions on visualization and computer graphics·2026

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Published on: September 23, 2012

Neurophotonics beyond the Surface: Unmasking the Brain's Complexity Exploiting Optical Scattering.

Fei Xia¹, Caio Vaz Rimoli^1,2, Walther Akemann²

¹Laboratoire Kastler Brossel, ENS-Université PSL, CNRS, Sorbonne Université, Collège de France, 24 rue Lhomond, 75005 Paris, France.

|April 2, 2024

Summary

Complex media optics can improve neuroimaging by overcoming light scattering in the brain. This approach enhances optical recordings and structural imaging for better understanding of neuronal activity.

Keywords:

brain probing complex media computational imaging neurophotonics wavefront shaping

More Related Videos

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Published on: May 7, 2017

Simultaneous Data Collection of fMRI and fNIRS Measurements Using a Whole-Head Optode Array and Short-Distance Channels

Simultaneous Data Collection of fMRI and fNIRS Measurements Using a Whole-Head Optode Array and Short-Distance Channels

Published on: October 20, 2023

Related Experiment Videos

Last Updated: Jul 7, 2026

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Published on: September 23, 2012

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Published on: May 7, 2017

Simultaneous Data Collection of fMRI and fNIRS Measurements Using a Whole-Head Optode Array and Short-Distance Channels

Simultaneous Data Collection of fMRI and fNIRS Measurements Using a Whole-Head Optode Array and Short-Distance Channels

Published on: October 20, 2023

Area of Science:

Neuroscience
Biomedical Optics
Physics

Background:

Studying the brain requires advanced techniques like neurophotonics for minimally invasive cellular and molecular probing.
Current neurophotonic methods face challenges in imaging depth, field of view, speed, and biocompatibility, largely due to light scattering in brain tissue.

Approach:

This perspective explores complex media optics, focusing on light propagation in heterogeneous materials, to enhance neuronal readouts.
Key strategies involve wavefront shaping and computational imaging/sensing techniques that leverage scattering properties.

Key Points:

Complex media optics offers solutions to overcome light scattering limitations in neuroimaging.
Wavefront shaping and computational methods are crucial for enhanced optical recordings and structural imaging.
Merging complex media optics with neurophotonics holds significant potential for advanced in vivo applications.

Conclusions:

Complex media optics can significantly advance neurophotonics by improving imaging depth, speed, and resolution.
Further research into merging these fields is essential for developing next-generation brain-probing technologies.