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Related Experiment Video

Updated: Jun 23, 2026

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Published on: February 17, 2015

A modular platform to generate functional sympathetic neuron-innervated heart assembloids.

Nadja Zeltner¹, Hsueh-Fu Wu¹, Kenyi Saito-Diaz¹

¹Universtiy of Georgia.

Research Square

|April 2, 2024

Summary

Researchers developed human sympathetic cardiac assembloids (hSCAs) by co-culturing sympathetic neurons with cardiac organoids. These innervated cardiac models exhibit mature structures and functional neural regulation, advancing disease modeling and drug discovery.

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Area of Science:

Stem cell biology
Neuroscience
Cardiovascular research

Background:

Human pluripotent stem cell (hPSC)-based organoids are crucial for studying development, disease, and drug discovery.
Most current organoids lack innervation, limiting their ability to model neural regulation of organ function and tissue maturation.
The autonomic sympathetic nervous system plays a vital role in regulating cardiac function.

Approach:

Developed a bioengineering-free method to create sympathetic neuron (symN)-innervated cardiac assembloids (hSCAs).
Utilized human pluripotent stem cells to generate both cardiac and sympathetic neuron components.
Integrated symNs with cardiac assembloids to achieve functional neural innervation.

Key Points:

hSCAs exhibit mature cardiac muscle structures, atrial-to-ventricular patterning, and spontaneous beating.
Innervating symNs demonstrate neurotransmitter synthesis and pharmacologically/optogenetically controllable regulation of cardiac beating rate.
Successfully modeled symN-mediated cardiac development and myocardial infarction in the hSCA system.

Conclusions:

hSCAs provide a novel, versatile platform for studying neuro-cardiac interactions and modeling cardiac diseases.
This model system is suitable for future neurocardiotoxicity screening and can be adapted for other organoid-neuronal combinations.
The modular nature allows for interchangeable neuron and organoid types, expanding research possibilities.