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Updated: Aug 13, 2026
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Published on: March 7, 2019
Genetic analysis of tumorigenesis: XXI. Suppressor genes in CHEF cells
Abstract:
In previous studies, fusions of transformed X nontransformed CHEF cells have produced hybrids that were suppressed for transformed traits and for tumor formation. During subsequent growth, the suppressed phenotypes were lost coincident with chromosome loss, and in one study the loss of anchorage dependence was correlated with loss of chromosome 1. In this paper, suppression of serum and anchorage requirements for growth is examined with the use of double-mutant tester stocks. Nontumorigenic low serum mutants from CHEF/18 cells are shown to complement with the lowered serum requirement of CHEF/16, a tumorigenic line, indicating that the high serum requirement is dominant and regulated by at least two genes. Similar results were previously reported for the anchorage requirement. Suppression of the two traits is found to segregate independently in hybrid subclones with reduced chromosome numbers, showing that different genes control suppression of the serum and anchorage requirements. Evidence for two modes of suppression, by dominant alleles of transformation genes and by unrelated genes, is presented and discussed.
Insights
Hybrid cell studies reveal that suppressing cancer traits like high serum and anchorage dependence involves distinct genetic mechanisms. These traits are regulated by different genes, with suppression occurring through dominant alleles or unrelated genes.
Area of Science:
- Cell biology
- Cancer research
- Genetics
Background:
- Hybrid cell studies previously showed suppression of transformed traits and tumor formation.
- Phenotype suppression was lost with chromosome loss, with anchorage dependence linked to chromosome 1 loss.
Purpose of the Study:
- To investigate the genetic basis of suppressed serum and anchorage requirements for growth in hybrid cells.
- To determine if different genes regulate the suppression of serum and anchorage dependence.
Main Methods:
- Utilized double-mutant tester stocks of CHEF/18 and CHEF/16 cell lines.
- Analyzed complementation of serum requirements between tumorigenic and nontumorigenic mutants.
- Examined segregation of suppressed traits in hybrid subclones with varying chromosome numbers.
Main Results:
- High serum requirement is dominant and regulated by at least two genes.
- Suppression of serum and anchorage requirements segregated independently in hybrid subclones.
- Identified distinct genetic controls for serum and anchorage suppression.
Conclusions:
- Serum and anchorage requirements are controlled by different genes.
- Two modes of suppression exist: dominant transformation gene alleles and unrelated genes.
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