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Updated: Jun 29, 2025

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
A ratiometric upconversion nanoprobe enables super-resolution imaging sensing of biothiols in living cells
Hongqi Chen1, Ruoxin Wang1, Juanjuan Cheng1
1Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, China. hq80chen@mail.ahnu.edu.cn.
Abstract:
We have developed an upconversion luminescent ratiometric nanoprobe, specifically designed for detection of biothiols with high sensitivity (∼25 nM) at the single-particle level. Using a single-particle localization and rendering method, this nanoprobe enables super-resolution imaging sensing of biothiols within a confined 22 nm space in living cells.
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