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Metabolomic and genomic insights into TMA degradation by a novel halotolerant strain - Paracoccus sp. PS1

Madhupa Seth1, Priyajit Mondal1, Dhritishree Ghosh1

  • 1Department of Microbiology, The University of Burdwan, Burdwan, Purba Bardhaman, 713104, West Bengal, India.

Archives of Microbiology
|April 2, 2024
PubMed

Insights

Researchers isolated a novel bacterial strain, Paracoccus sp. PS1, capable of degrading trimethylamine (TMA). This discovery offers a potential probiotic strategy to reduce trimethylamine N-oxide (TMAO) levels in the human gut, mitigating chronic disease risks.

Area of Science:

  • Microbiology
  • Gut Microbiome Research
  • Metabolic Engineering

Background:

  • Trimethylamine N-oxide (TMAO) is a metabolite linked to chronic diseases.
  • TMAO is produced by the oxidation of trimethylamine (TMA), generated by gut bacteria.
  • Reducing TMA production is a strategy to limit TMAO formation.

Purpose of the Study:

  • To isolate and characterize bacterial strains capable of degrading TMA.
  • To identify a potential probiotic for reducing TMAO generation in the gut.

Main Methods:

  • Isolation of TMA-degrading bacteria from marine fish skin.
  • Screening isolates based on TMA utilization.
  • Confirmation of TMA degradation using spectrophotometry, ESI TOF-MS, and HPLC.
  • Whole genome sequencing and 16S rRNA analysis for taxonomic identification and pathway elucidation.

Main Results:

  • Fourteen TMA-degrading isolates were obtained from fish skin.
  • Isolate PS1 demonstrated rapid TMA utilization and degradation.
  • Genomic analysis identified PS1 as Paracoccus sp. and revealed TMA degradation pathways.
  • The isolate possesses enzymes crucial for TMA breakdown.

Conclusions:

  • Paracoccus sp. PS1 is an effective TMA degrader.
  • This isolate holds potential for clinical applications to reduce gut TMAO production.
  • Further research may lead to novel probiotic interventions for TMAO-related diseases.
Keywords:
AtherosclerosisMicrobiota, Paracoccus sp.Trimethylamine (TMA)Trimethylamine N-oxide (TMAO)

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