Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Area of Science:

• Electrochemistry
• Materials Science
• Biochemistry

Background:

• Glycoproteins are crucial biomarkers in clinical diagnosis.
• Developing sensitive and specific biosensing platforms for glycoproteins is essential.
• Metal-organic frameworks (MOFs) offer unique properties for biosensor development.

Purpose of the Study:

• To establish an electrochemical impedimetric biosensing platform for sensitive glycoprotein detection.
• To utilize MIL-101(Cr)-NH2 as a supporting matrix for lectin immobilization.
• To determine invertase (INV) as a model glycoprotein using the fabricated biosensor.

Main Methods:

• Immobilization of concanavalin A (Con A) lectin onto MIL-101(Cr)-NH2.
• Electrochemical impedance spectroscopy (EIS) for signal transduction.
• Optimization of biosensor parameters including Con A concentration and incubation times.
• Testing with varying concentrations of invertase (INV) and analysis in real mouse serum samples.

Main Results:

• The Con A@MIL-101(Cr)-NH2 biosensor demonstrated a linear response to INV concentration from 1.0 × 10^-16 to 1.0 × 10^-11 M.
• A highly sensitive limit of detection (LOD) of 3.98 × 10^-18 M was achieved for INV.
• The biosensor exhibited excellent specificity, stability, reproducibility, and repeatability.
• High recovery rates were observed when analyzing INV in spiked mouse serum samples.

Conclusions:

• The developed electrochemical impedimetric biosensing platform shows great potential for sensitive and reliable glycoprotein detection.
• The use of MIL-101(Cr)-NH2 as a matrix enhances lectin immobilization and biosensor performance.
• The platform's ability to analyze glycoproteins in complex biological matrices like serum highlights its clinical diagnostic applicability.