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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 6, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

Microarray Platforms Based on 3D Printing.

Jinglin Qin¹, Zhenwei Qian², Yiwen Lai¹

¹Department of Neurobiology, School of Basic Medical Sciences, Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China.

Analytical Chemistry

|April 3, 2024

Summary

This summary is machine-generated.

This study presents a novel digital light processing (DLP) 3D printing method for creating precise microwell arrays and microfluidic devices. This 3D printing technique enables advanced spheroid generation and drug screening assays with modulated drug combinations.

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Area of Science:

Microfluidics
3D Printing
Biotechnology

Background:

Microwell arrays are crucial for high-throughput biological assays.
Existing fabrication methods can be costly and lack flexibility.
Need for advanced microfluidic devices for complex biological studies.

Purpose of the Study:

To introduce a low-cost, high-precision digital light processing (DLP) 3D printing method for fabricating microwell arrays and microfluidic devices.
To demonstrate the capability of this method for generating spheroid flowcells and performing complex biological assays.
To explore the impact of drug combination timing on therapeutic outcomes.

Main Methods:

Fabrication of microwell arrays and microchannels using a low-cost DLP 3D printer.
Integration of microchannels with microwell arrays for liquid transfer and microdroplet dispensing.
Generation of spheroid flowcells using micropyramid arrays and parallel microchannels.
Performance of parallel drug dose-response assays and dynamic drug combination studies.

Main Results:

Successful fabrication of microwell arrays (200-2000 μm diameter) and microchannels (45-1000 μm width) with high precision.
Demonstrated chip-to-chip dispensing for multistep and multireagent assays.
Generated and manipulated spheroids in integrated spheroid flowcells.
Validated the impact of drug combination timing and sequence on therapeutic outcomes in parallel assays.

Conclusions:

DLP 3D printing offers a flexible and cost-effective approach for advanced microfluidic device fabrication.
The developed platform enables precise control over spheroid generation and microenvironment modulation.
This technology facilitates complex biological assays, including dynamic drug combination studies, with significant implications for drug discovery and personalized medicine.