Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toxic and useful: harnessing yeast killer toxins for product recovery.

Microbial cell factories·2026
Same author

PYEAST - A Computational Toolkit for Saccharomyces cerevisiae Genetic Engineering.

NPJ systems biology and applications·2026
Same author

Designing Functional Membranes with Tunable PDA/PEI Coatings for Enzyme Entrapment.

ACS applied materials & interfaces·2026
Same author

Atomic Force Microscopy Infrared Spectroscopy Method for Multisample Comparison of Topographic, Infrared Imaging, and Stiffness Domains.

Analytical chemistry·2025
Same author

An Approach to Intraoperative Accidental Pulmonary Artery Transection and Role of Mid-Point to Transverse Process Interfascial Plane Block.

Annals of cardiac anaesthesia·2025
Same author

Phase plane analysis and novel soliton solutions for the space-time fractional Boussinesq equation using two robust techniques.

PloS one·2025

Related Experiment Video

Updated: Jun 29, 2025

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
08:43

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Published on: July 17, 2019

8.0K

Functionalizing Yeast Lipid Droplets as Versatile Biomaterials.

Ankita Suri1,2, Kevin K Y Hu1, Tayyaba Younas1

  • 1Department of Chemical and Biological Engineering, Monash University, Clayton, 3800, Australia.

Small (Weinheim an Der Bergstrasse, Germany)
|April 3, 2024
PubMed
Summary

Engineered yeast lipid droplets (LD) serve as versatile biomaterials. These modified LD display proteins for reporting, binding, membrane fusion, and catalysis, extending their function beyond lipid storage.

Keywords:
atomic force microscopybiocatalysisbiosensorcell cargo transportflow cytometryfluorescent proteinsuper‐resolution confocal microscopy

More Related Videos

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
10:22

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Published on: April 3, 2018

7.6K
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

19.7K

Related Experiment Videos

Last Updated: Jun 29, 2025

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
08:43

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Published on: July 17, 2019

8.0K
Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
10:22

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Published on: April 3, 2018

7.6K
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

19.7K

Area of Science:

  • Cell Biology
  • Biomaterials Science
  • Protein Engineering

Background:

  • Lipid droplets (LD) are cellular organelles primarily for lipid storage.
  • Engineering LD offers potential for novel biomaterial applications.
  • Current engineering strategies for LD are underdeveloped.

Purpose of the Study:

  • To engineer yeast Saccharomyces cerevisiae lipid droplets (LD) to display functional proteins.
  • To explore the potential of engineered LD as versatile biomaterials.
  • To demonstrate the stability and functionality of proteins anchored to engineered LD.

Main Methods:

  • Genetic engineering of yeast to create fusion proteins linking oleosin to mCherry, Halotag, v-SNARE, and carbonic anhydrase.
  • Expression and specific display of fusion proteins on LD.
  • Assessment of engineered LD functions using fluorescence confocal microscopy, TEM, AFM, flow cytometry, spectrophotometry, and enzyme activity assays.

Main Results:

  • Engineered LD successfully displayed multiple functional proteins (mCherry, Halotag, v-SNARE, carbonic anhydrase) via oleosin fusion.
  • Isolated LD were robust and stabilized the anchored proteins.
  • Engineered LD demonstrated capabilities as reporters, ligand binders, membrane-targeting agents, and enzyme catalysts.

Conclusions:

  • Engineered LD can be developed into functional biomaterials with applications beyond lipid storage.
  • The oleosin platform provides a versatile method for anchoring and displaying proteins on LD.
  • This approach expands the utility of LD for various cellular and material science applications.