Related Experiment Video

Updated: Jun 29, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.2K

Asiatic acid inhibits osteosarcoma cell migration and invasion via the AKT/Sp1/MMP1 axis

Yat-Yin Law1,2,3, Hsiang-Lin Lee1,4, Chu-Liang Lin3

  • 1School of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Environmental Toxicology
|April 3, 2024
PubMed

Insights

Asiatic acid (AA) shows potential against osteosarcoma by inhibiting cancer cell migration and invasion. It targets the p-AKT/Sp1/MMP1 pathway, offering a new avenue for treating this bone cancer.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Osteosarcoma is a prevalent bone cancer in children and adolescents with limited treatment options.
  • Asiatic acid (AA), derived from Centella asiatica, exhibits known anti-tumor properties in various cancer cell types.
  • Investigating novel therapeutic agents for osteosarcoma is crucial due to the lack of effective treatments.

Purpose of the Study:

  • To evaluate the antitumor effects of Asiatic acid (AA) on human osteosarcoma cells.
  • To elucidate the molecular mechanisms underlying AA's action in osteosarcoma.
  • To explore the potential of AA as a therapeutic agent for osteosarcoma.

Main Methods:

  • Cell viability, proliferation, and cell cycle assays were performed.
  • Cell migration and invasion assays were conducted.
  • Gene expression analysis (MMP1, Sp1) and Western blotting (p-AKT) were utilized.
  • Bioinformatic analysis of the TNMplot database was performed.

Main Results:

  • Asiatic acid (AA) did not affect osteosarcoma cell viability, proliferation, or cell cycle.
  • AA significantly suppressed osteosarcoma cell migration and invasion by down-regulating MMP1 expression.
  • AA reduced Sp1 expression and AKT phosphorylation, key components of the p-AKT/Sp1/MMP1 pathway.
  • MMP1 overexpression reversed AA's anti-migratory and anti-invasive effects, while Sp1 overexpression abolished AA's efficacy.

Conclusions:

  • Asiatic acid (AA) demonstrates anti-invasive and anti-migratory effects on human osteosarcoma cells.
  • The anti-cancer activity of AA is mediated through the p-AKT/Sp1/MMP1 signaling pathway.
  • AA holds promise as a potential therapeutic candidate for osteosarcoma treatment.
Keywords:
AKTMMP1Sp1asiatic acidosteosarcoma

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