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Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen
Zili Gao1, Shenglong Ding2, Tingting Fan1
1Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China.
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Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling.
