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Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Protocol For Identifying Stressed Granulocytes From Septic Mice.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Protocol For Identifying Stressed Granulocytes From Septic Mice.

Related Experiment Video

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Protocol for identifying stressed granulocytes from septic mice.

Yu Hao1, Can Zhang1, Fangyuan Li1

  • 1Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China; Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing 100038, China.

STAR Protocols
|April 3, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Sepsis primes immune cells called granulocytes, enhancing their response to subsequent infections. This study details a mouse model to investigate this phenomenon and its impact on inflammation and survival.

Keywords:
Flow CytometryImmunologyModel Organisms

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Area of Science:

  • Immunology
  • Microbiology
  • Infectious Diseases

Background:

  • Sepsis can alter immune cell function, potentially affecting responses to subsequent infections.
  • Granulocytes play a critical role in the innate immune response to bacterial pathogens.

Purpose of the Study:

  • To present a reproducible murine model for studying sepsis followed by a secondary infection.
  • To investigate the functional changes in granulocytes following sepsis and their impact on host defense.

Main Methods:

  • Establishment of a murine model using cecal ligation and puncture (CLP) to induce sepsis.
  • Rechallenging mice with lipopolysaccharide (LPS) or Pseudomonas aeruginosa during the recovery phase.
  • Characterization of granulocyte phenotypes and assessment of their functional capacity.

Main Results:

  • The developed model allows for the study of sepsis-induced immune training in granulocytes.
  • Sepsis-conditioned granulocytes exhibit altered functional phenotypes.
  • The functional state of granulocytes influences the mortality of mice facing secondary infections.

Conclusions:

  • This protocol provides a valuable tool for investigating the complex interplay between sepsis, granulocyte function, and secondary infections.
  • Understanding sepsis-induced immune modulation in granulocytes is crucial for developing targeted therapies to improve patient outcomes.