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Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Related Experiment Video

Updated: Apr 11, 2026

An Innovative Method for Exosome Quantification and Size Measurement

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

Size-Selective Capturing of Exosomes Using DNA Tripods.

Ryosuke Iinuma^1,2, Xiaoxia Chen³, Takeya Masubuchi^1,4

¹Graduate School of Frontier Science, The University of Tokyo, Chiba 277-8562, Japan.

Journal of the American Chemical Society

|April 3, 2024

Summary

Researchers developed a DNA origami tripod nanodevice for efficient purification of extracellular vesicles (EVs). This method simplifies EV isolation from small samples by simultaneously selecting based on size and biomarkers.

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Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

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Last Updated: Apr 11, 2026

An Innovative Method for Exosome Quantification and Size Measurement

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Published on: April 13, 2018

Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Fractionating biomolecules is crucial for analysis.
Extracellular vesicles (EVs) hold diagnostic potential but require complex purification.
Conventional methods limit analysis of small biological samples.

Purpose of the Study:

To develop a simplified method for purifying extracellular vesicles (EVs).
To enable precise analysis of biomolecules from limited sample volumes.
To demonstrate size- and biomarker-based EV selection using nanotechnology.

Main Methods:

Utilized a DNA origami tripod nanodevice functionalized with antibodies.
Employed the nanodevice to capture exosomes (a type of EV) from crude mixtures.
Investigated control over captured EV size by adjusting tripod aperture.

Main Results:

The DNA origami tripod successfully captured specific sizes of EVs based on biomarker recognition.
Demonstrated selective isolation of EVs from complex biological mixtures.
Showcased tunable size selection of EVs by modifying the nanodevice aperture.

Conclusions:

The DNA origami tripod offers a simplified and precise method for EV purification.
This approach facilitates the analysis of EVs and other target biomolecules from small samples.
Nanotechnology-based selective capture advances biomolecular analysis and diagnostics.