Related Experiment Video
Updated: May 2, 2026
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
Isolation and identification of a novel phage targeting clinical multidrug-resistant Corynebacterium striatum
Jiao Wang1, Meng Zhang1, Jiao Pei2
1Department of Pathogen Biology, School of Basic Medicine, Hubei University of Arts and Science, Xiangyang, China.
Introduction:
Over the past decade, Corynebacterium striatum (C. striatum), an emerging multidrug-resistant (MDR) pathogen, has significantly challenged healthcare settings, especially those involving individuals with weakened immune systems. The rise of these superbugs necessitates innovative solutions.
Methods:
This study aimed to isolate and characterize bacteriophages targeting MDR-C. striatum. Utilizing 54 MDR-C. striatum isolates from a local hospital as target strains, samples were collected from restroom puddles for phage screening. Dot Plaque and Double-layer plate Assays were employed for screening.
Results:
A novel temperate bacteriophage, named CSP1, was identified through a series of procedures, including purification, genome extraction, sequencing, and one-step growth curves. CSP1 possesses a 39,752 base pair circular double-stranded DNA genome with HK97-like structural proteins and potential for site-specific recombination. It represents a new species within the unclassified Caudoviricetes class, as supported by transmission electron microscopy, genomic evolutionary analysis, and collinearity studies. Notably, CSP1 infected and lysed 21 clinical MDR-C. striatum isolates, demonstrating a wide host range. The phage remained stable in conditions ranging from -40 to 55°C, pH 4 to 12, and in 0.9% NaCl buffer, showing no cytotoxicity.
Discussion:
The identification of CSP1 as the first phage targeting clinical C. striatum strains opens new possibilities in bacteriophage therapy research, and the development of diagnostic and therapeutic tools against pathogenic bacteria.
Insights
Researchers discovered CSP1, a novel bacteriophage effective against multidrug-resistant Corynebacterium striatum (MDR-C. striatum). This finding offers a promising new avenue for bacteriophage therapy and combating superbug infections in healthcare settings.
Area of Science:
- Microbiology
- Virology
- Infectious Diseases
Background:
- Corynebacterium striatum (C. striatum) is an emerging multidrug-resistant (MDR) pathogen causing significant healthcare challenges, particularly in immunocompromised individuals.
- The increasing prevalence of MDR pathogens necessitates the development of novel therapeutic strategies.
Purpose of the Study:
- To isolate and characterize bacteriophages capable of targeting multidrug-resistant Corynebacterium striatum (MDR-C. striatum).
- To evaluate the potential of identified bacteriophages for therapeutic applications against C. striatum infections.
Main Methods:
- Screening of bacteriophages from environmental samples (restroom puddles) against 54 clinical MDR-C. striatum isolates.
- Purification, genome extraction, sequencing, and characterization of the isolated bacteriophage CSP1.
- Assays included Dot Plaque, Double-layer plate, one-step growth curves, transmission electron microscopy, and stability tests (temperature, pH, salinity).
Main Results:
- A novel temperate bacteriophage, CSP1, was identified with a 39,752 bp double-stranded DNA genome, classified as a new species within the Caudoviricetes class.
- CSP1 demonstrated a broad host range, infecting and lysing 21 clinical MDR-C. striatum isolates.
- The bacteriophage exhibited stability across a wide range of temperatures (-40 to 55°C), pH (4 to 12), and salinity, with no observed cytotoxicity.
Conclusions:
- CSP1 is the first identified phage targeting clinical Corynebacterium striatum strains.
- This discovery presents significant potential for bacteriophage therapy development against MDR-C. striatum.
- The findings pave the way for novel diagnostic and therapeutic tools to combat pathogenic bacteria.
More Related Videos
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
09:01Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
Published on: September 28, 2022
Related Concept Videos
01:24Techniques for Isolation of Pure Cultures
01:28Methods of Classification and Identification