Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Validation of a Questionnaire Measuring Treatment Burden in Pediatric Asthma.

The journal of allergy and clinical immunology. In practice·2025
Same author

Iptacopan for Immune Thrombocytopenia and Cold Agglutinin Disease: A Global Phase 2 Basket Clinical Trial.

American journal of hematology·2025
Same author

A Streamlined Miniaturized and Multi-Allergen Basophil Activation Test Platform Amenable to Population-Based Study.

Allergy·2025
Same author

Cryopreservation Between Sample Processing and Analysis in Streamlined Basophil Activation Test.

Allergy·2025
Same author

Perfluorobutane Nanodroplets for the Selective Sensing and Range Verification of Carbon-Ion Radiotherapy: In Vitro Evaluation on Cells.

ACS omega·2025
Same author

Oral iptacopan monotherapy in paroxysmal nocturnal haemoglobinuria: final 48-week results from the open-label, randomised, phase 3 APPLY-PNH trial in anti-C5-treated patients and the open-label, single-arm, phase 3 APPOINT-PNH trial in patients previously untreated with complement inhibitors.

The Lancet. Haematology·2025

Related Experiment Video

Updated: Jun 29, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.2K

Possible alternative strategies to implement basophil activation testing in multicentric studies.

Pénélope Bourgoin1, Thomas Dupont1, Chantal Agabriel2

  • 1Global Research Organization, Beckman Coulter Life Sciences, Marseille, France.

Cytometry. Part B, Clinical Cytometry
|April 4, 2024
PubMed
Summary

The Basophil Activation Test (BAT) can be adapted for multicentric studies. Processing blood before storage offers greater flexibility and stability for basophil reactivity testing up to seven days.

Keywords:
basophil activation testingbasophil reactivityblood sample stabilityflow cytometryinterleukin‐3multicentric studies

More Related Videos

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.6K
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
11:55

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

Published on: March 14, 2011

14.6K

Related Experiment Videos

Last Updated: Jun 29, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.2K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.6K
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
11:55

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

Published on: March 14, 2011

14.6K

Area of Science:

  • Immunology
  • Allergy Diagnostics
  • Flow Cytometry

Background:

  • The Basophil Activation Test (BAT) is crucial for characterizing basophil reactivity to allergens using flow cytometry.
  • Implementing BAT in multicentric studies faces challenges due to blood sample stability.
  • Previous simplified approaches necessitate further evaluation for broader application.

Purpose of the Study:

  • To evaluate two distinct strategies for implementing BAT in multicentric studies: storing whole blood versus processed samples.
  • To assess the impact of storage conditions (temperature, duration) and anticoagulants on basophil reactivity.
  • To determine the feasibility of different BAT workflows for decentralized clinical trials.

Main Methods:

  • Compared two BAT workflows: 'collect, store, process & analyze' and 'collect, process, store & analyze'.
  • Utilized heparin- and EDTA-anticoagulated whole blood samples.
  • Investigated storage at 18-25°C and 2-8°C for up to 7 days, evaluating interleukin-3's role.

Main Results:

  • The 'collect, store, process & analyze' workflow showed optimal results with heparinized blood stored at 18-25°C, though reactivity decreased after 24 hours.
  • Interleukin-3 did not enhance basophil reactivity after storage in tested conditions.
  • The 'collect, process, store & analyze' workflow allowed storage of processed blood (heparin or EDTA) for up to 7 days at either temperature without impacting reactivity.

Conclusions:

  • The 'collect, process, store & analyze' workflow provides significant flexibility for multicentric BAT implementation without compromising basophil reactivity.
  • The 'collect, store, process & analyze' workflow is logistically simpler but requires caution due to potential reactivity decay.
  • Both strategies can be adapted for multicentric studies, with the choice depending on logistical constraints and required sample stability.