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Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
Published on: July 24, 2018
Application of the surface engineered recombinant Escherichia coli to the industrial battery waste solution for
Jaehoon Jeong1, Vidhya Selvamani1, Murali Kannan Maruthamuthu1
1Department of Chemical Engineering, University of Ulsan, Namgu, Ulsan 44610, Republic of Korea.
Abstract:
Escherichia coli were engineered to selectively adsorb and recover lithium from the environment by employing a bacterial cell surface display strategy. Lithium binding peptide (LBP1) was integrated into the Escherichia coli membrane protein OmpC. The effect of environmental conditions on the adsorption of lithium by a recombinant strain was evaluated, and lithium particles on the cellular surface were analyzed by FE-SEM and XRD. To elevate the lithium adsorption, dimeric, trimeric, and tetrameric repeats of the LBP1 peptide were constructed and displayed on the surface of E. coli. The constructed recombinant E. coli displaying the LBP1 trimer was applied to real industrial lithium battery wastewater to recover lithium.
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