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Infectivity of Friend murine leukemia virus for hamster cells
Abstract:
Hamster-adapted Friend murine leukemia virus (F-MuLV) infected hamster cells and produced an XC-positive progeny. However, the sensitivity of hamster-adapted F-MuLV's to hamster cells was about 100 times lower than that to mouse cells. Hamster cells chronically infected with F-MuLV in vitro produced progeny virus in titers comparable to those in similarly infected mouse cells. Xenotropic and amphotropic murine leukemia virus can infect hamster cells when phenotypically mixed with the F-MuLV. Murine sarcoma virus pseudotyped with the F-MuLV easily induced sarcomas in hamsters and transformed hamster cells in vitro.
Insights
Hamster-adapted Friend murine leukemia virus (F-MuLV) infects hamster cells but is less sensitive than in mouse cells. However, chronic infection yields comparable virus titers, and F-MuLV can facilitate other murine leukemia virus infections in hamsters.
Area of Science:
- Virology
- Oncology
- Cell Biology
Background:
- Friend murine leukemia virus (F-MuLV) is a retrovirus known to infect rodent cells.
- Understanding F-MuLV tropism and infectivity in different host cells is crucial for viral pathogenesis research.
- Previous studies have focused on F-MuLV in mouse models, with limited data on hamster cell interactions.
Purpose of the Study:
- To investigate the infectivity and replication of hamster-adapted F-MuLV in hamster cells.
- To compare the sensitivity of hamster cells to F-MuLV with that of mouse cells.
- To explore the potential of F-MuLV in facilitating the infection of other murine leukemia viruses in hamster cells.
Main Methods:
- Infection of hamster cells with hamster-adapted F-MuLV.
- Quantification of XC-positive progeny virus.
- Comparison of viral sensitivity between hamster and mouse cells.
- Phenotypic mixing experiments with xenotropic and amphotropic murine leukemia viruses.
- In vitro transformation assays using murine sarcoma virus pseudotyped with F-MuLV.
Main Results:
- Hamster-adapted F-MuLV infected hamster cells, producing XC-positive progeny.
- Hamster cells exhibited approximately 100-fold lower sensitivity to hamster-adapted F-MuLV compared to mouse cells.
- Chronic infection of hamster cells with F-MuLV resulted in comparable progeny virus titers to infected mouse cells.
- Xenotropic and amphotropic murine leukemia viruses could infect hamster cells via phenotypic mixing with F-MuLV.
- Murine sarcoma virus pseudotyped with F-MuLV efficiently induced sarcomas in hamsters and transformed hamster cells in vitro.
Conclusions:
- Hamster cells are permissive to F-MuLV infection, albeit with reduced sensitivity compared to mouse cells.
- F-MuLV can act as a helper virus to enable infection by other murine leukemia viruses in hamster cells.
- F-MuLV-pseudotyped murine sarcoma virus demonstrates oncogenic potential in hamsters, highlighting F-MuLV's role in oncogenesis.