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Updated: May 9, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT
Myeongbin Kim1, Ryun Kang1, Tae Jin Jeon2
1Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04673, Republic of Korea.
Abstract:
The microbial transcription factor YhaJ responds to 2,4-dinitrotoluene (DNT) derivatives. Here, we describe steps for overexpression and purification of the protein, characterization for the binding of a DNT derivative methylhydroquinone, and crystallization by using a random seeding technique. We then detail procedures for structure determination by employing the crystal-twin resolving processes. This protocol can also be performed using other DNT derivatives. For complete details on the use and execution of this protocol, please refer to Kim et al.1.
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