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Updated: Jul 31, 2026
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DNA-based Fish Species Identification Protocol
Published on: April 28, 2010
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Genetic identification of three CITES-listed sharks using a paper-based Lab-on-a-Chip (LOC).
Guuske P Tiktak1, Alexandria Gabb1, Margarita Brandt2,3
1Ecology & Environment Research Centre, Manchester Metropolitan University, Manchester, United Kingdom.
Plos One
|April 4, 2024
Summary
A new paper-based Lab-on-a-Chip (LOC) device enables rapid, field-based identification of threatened shark species. This technology aids in monitoring fisheries and combating illegal wildlife trade by providing simple results without specialist equipment.
Area of Science:
- Marine Biology
- Conservation Science
- Biotechnology
Background:
- Threatened shark species are frequently caught and traded globally.
- Current species identification methods (expert visual, DNA barcoding) require specialized labs and personnel, limiting market monitoring.
- Effective monitoring is crucial for reducing illegal fishing and conserving CITES-restricted shark products.
Purpose of the Study:
- To develop a field-deployable method for identifying threatened shark species at the point of sale.
- To create a user-friendly tool for non-specialists to identify bigeye thresher, pelagic thresher, and shortfin mako sharks.
- To establish a proof-of-concept for accessible species identification in fisheries and seizure contexts.
Main Methods:
- Development of a paper-based Lab-on-a-Chip (LOC) device.
- DNA extraction from shark meat and fin samples.
- DNA amplification and visualization using Loop Mediated Isothermal Amplification (LAMP) on the LOC.
- Integration of positive and negative controls for reliable results.
Main Results:
- Successful DNA extraction from various shark sample types.
- Rapid (under one hour) identification of three target threatened shark species (bigeye thresher, pelagic thresher, shortfin mako).
- Clear "yes/no" visual result (color change from pink to yellow) indicating species presence.
Conclusions:
- The paper-based LOC with LAMP is a viable tool for field-based shark species identification.
- This technology can be operated by non-scientifically trained personnel, overcoming limitations of current methods.
- The LOC facilitates monitoring in markets with high mislabeling rates and aids in identifying dried shark fins during seizures, supporting conservation efforts.
