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Dynamic responses of Fusarium mangiferae to ultra-violet radiation

Shira Milo1, Ritah Namawejje2, Roi Krispin2

  • 1Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment. the Hebrew University of Jerusalem, Israel; Department of Natural and Life Sciences, The Open University of Israel, Israel.

Fungal Biology
|April 4, 2024
PubMed

Insights

Foliar fungi like Fusarium mangiferae show no developmental control over UV DNA repair, unlike soil fungi. UV exposure primarily induces translation-related genes during recovery.

Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Fungal adaptation to diverse environments relies on DNA repair mechanisms, particularly for ultra-violet (UV) damage.
  • Previous research indicated developmental regulation of UV repair in the soil-borne fungus Fusarium oxysporum.
  • This study investigates UV DNA damage response in the foliar pathogen Fusarium mangiferae.

Purpose of the Study:

  • To determine if Fusarium mangiferae exhibits developmental control over UV repair, photolyase gene expression, and UV-induced mutagenesis.
  • To identify genes and pathways involved in the recovery of F. mangiferae following UV irradiation.

Main Methods:

  • Comparative analysis of UV survival, photoreactivation-dependent repair, and mutagenesis across developmental stages of F. mangiferae.
  • RNA sequencing (RNAseq) analysis of F. mangiferae post-UV irradiation at various time points.
  • Gene expression analysis of photolyase.

Main Results:

  • F. mangiferae lacks developmental control over photoreactivation-dependent UV repair and photolyase gene expression, contrasting with F. oxysporum.
  • UV-induced mutagenesis reduction via photoreactivation was consistent across F. mangiferae developmental stages.
  • UV exposure significantly induced translation-related genes in a development-dependent manner in F. mangiferae.

Conclusions:

  • The developmental regulation of UV DNA repair differs between foliar and soil-borne Fusarium species.
  • F. mangiferae's recovery from UV damage involves a complex, time-dependent response impacting translation, cell cycle, and lipid metabolism genes.

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