Osteosarcoma-targeted Cu and Ce based oxide nanoplatform for NIR II fluorescence/magnetic resonance dual-mode imaging...

Area of Science:

• Biomedical Engineering
• Materials Science
• Oncology

Background:

• Osteosarcoma is a lethal bone tumor common in children and adolescents, characterized by local destruction and high metastasis.
• There is an urgent need for advanced nanoplatforms offering high therapeutic efficacy and precise diagnosis for osteosarcoma.
• Multimodal optical imaging and programmed treatment strategies, like synergistic photothermal-chemodynamic therapy (PTT-CDT) inducing immunogenetic cell death (ICD), show promise for sensitive imaging and effective treatment with minimal side effects.

Purpose of the Study:

• To design and construct a mesoporous copper and cerium oxide nanoplatform for targeted osteosarcoma therapy and dual-mode bio-imaging.
• To evaluate the efficacy of the nanoplatform in ablating osteosarcoma through PTT-CDT and ICD stimulation.
• To assess the nanoplatform's capability as a contrast agent for near-infrared II (NIR II) fluorescence and magnetic resonance imaging (MRI) in osteosarcoma discrimination.

Main Methods:

• Synthesis of mesoporous copper and cerium oxide nanoparticles anchored with Arg-Gly-Asp (RGD) peptide for targeted delivery.
• Loading of indocyanine green (ICG) onto the nanoplatform for photothermal-chemodynamic therapy and fluorescence imaging.
• In vitro and in vivo evaluation of the nanoplatform's therapeutic efficacy against osteosarcoma.
• Utilizing the nanoplatform for dual-mode bio-imaging, including NIR II fluorescence and MRI, for osteosarcoma tissue discrimination.

Main Results:

• Successful construction of a RGD-anchored, mesoporous Cu&Ce oxide nanoplatform.
• The nanoplatform effectively targeted and ablated osteosarcoma cells in vitro and in vivo via PTT-CDT and ICD.
• The nanoplatform demonstrated dual-mode bio-imaging capabilities, providing NIR II fluorescence and MRI signals for enhanced osteosarcoma visualization.
• The RGD targeting moiety facilitated precise delivery and accumulation of the nanoplatform at the tumor site.

Conclusions:

• The developed Cu&Ce-based mesoporous nanoplatform shows significant potential for efficient osteosarcoma suppression.
• The nanoplatform enables dual-mode bio-imaging, offering a new avenue for accurate diagnosis and monitoring of malignant osteosarcoma.
• This study opens new possibilities for the clinical translation of targeted nanotheranostics for osteosarcoma treatment.