Ultrasound Trigger Ce-Based MOF Nanoenzyme For Efficient Thrombolytic Therapy

Area of Science:

• Biomaterials Science
• Nanomedicine
• Thrombosis Research

Background:

• Thrombus formation and dissolution cause inflammatory damage, increasing thrombotic complication risks.
• Existing thrombolytic therapies require strategies to simultaneously resist oxidation and inflammation.
• A rapid, precise, and dual-action therapeutic approach is crucial for managing thrombotic events.

Purpose of the Study:

• To develop a targeted nanomedicine, Ce-UiO-CM, for enhanced thrombolytic therapy.
• To investigate the reactive oxygen species (ROS) scavenging and anti-inflammatory properties of Ce-UiO-66.
• To evaluate the efficacy of Ce-UiO-CM combined with ultrasound in dissolving thrombi.

Main Methods:

• Constructed Ce-UiO-CM by encapsulating cerium-based metal-organic framework (Ce-UiO-66) within a mesenchymal stem cell membrane.
• Utilized Ce-UiO-66's catalase (CAT) activity to react with hydrogen peroxide (H2O2) and produce oxygen.
• Applied Ce-UiO-CM with external ultrasound stimulation for thrombolytic therapy in a rat femoral artery model.

Main Results:

• Ce-UiO-66 demonstrated significant ROS scavenging capacity due to its structure and Ce (III)/Ce (IV) coupling sites.
• The nanomedicine exhibited inflammation-targeting properties via the mesenchymal stem cell membrane.
• Ultrasound-enhanced cavitation effects, boosted by Ce-UiO-66's porous structure and oxygen production, improved thrombolysis efficacy.

Conclusions:

• Ce-UiO-CM is an effective targeted nanomedicine for thrombolytic therapy.
• The combination of Ce-UiO-66's ROS scavenging and ultrasound stimulation enhances thrombus dissolution.
• This novel therapeutic strategy holds potential for treating thrombotic complications with reduced inflammatory damage.