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Related Experiment Video

Updated: Jun 29, 2025

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

Affordable Method for Hematocrit Determination in Murine Models.

Siobhan Branfield¹, A Valance Washington¹, Barbara Manfredi¹

¹Department of Biological Sciences, Oakland University, Rochester Hills, Michigan.

Current Protocols

|April 5, 2024

Summary

This study presents an affordable method for determining hematocrit (Hct) in mouse models using a standard benchtop centrifuge. This accessible technique offers comparable accuracy to specialized equipment, aiding research labs with limited budgets.

Keywords:

anemia‐induced hematocrit (Hct)mouse models phenylhydrazine (PHZ)retro‐orbital bleeding (REB)

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Last Updated: Jun 29, 2025

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

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Published on: August 5, 2011

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

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Flow Cytometric Characterization of Murine B Cell Development

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Published on: January 22, 2021

Area of Science:

Hematology
Biomedical Research
Laboratory Methods

Background:

Hematocrit (Hct) is crucial for diagnosing anemia and serves as a key hematological parameter in research.
Standard Hct measurement requires expensive, specialized equipment like the CritSpin™ Microhematocrit Centrifuge.
Many research laboratories face budget constraints that limit access to specialized hematological equipment.

Purpose of the Study:

To develop and validate a novel, simple, and affordable method for determining Hct in murine models.
To compare the accuracy of the new method with a standardized, expensive technique.
To provide an accessible Hct measurement solution for research laboratories with limited financial resources.

Main Methods:

Utilized a commonly available benchtop centrifuge for processing murine blood samples.
Compared Hct results from the benchtop centrifuge method against the CritSpin™ Microhematocrit Centrifuge.
Employed phenylhydrazine (PHZ)-induced anemia in wild-type (WT) mice to validate the method across different hematological states.

Main Results:

The affordable benchtop centrifuge method yielded Hct results comparable to the standardized CritSpin™ method.
The novel approach demonstrated reasonable accuracy in determining Hct in both wild-type and anemic mouse models.
Validation confirmed the reliability of the method for murine sample analysis.

Conclusions:

An affordable and accessible method for Hct determination in murine models has been successfully established.
This technique offers a viable alternative for research laboratories lacking specialized hematocrit measurement equipment.
The developed protocol can facilitate hematological research in resource-limited settings.