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Related Experiment Videos

A micro method for enumerating T and B cells in mouse peripheral blood.

E Jarrett-Toth, D Segar, R Hong

    Journal of Immunological Methods
    |January 1, 1979
    PubMed
    Summary

    A new micro method accurately assesses T and B lymphocytes and monocytes in mouse blood. This technique uses minimal blood volume, avoiding cell loss for reliable immune cell analysis.

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    Area of Science:

    • Immunology
    • Hematology
    • Mouse Models

    Background:

    • Accurate assessment of immune cell populations is crucial in immunological research.
    • Traditional methods for analyzing T and B lymphocytes can be labor-intensive and may lead to cell loss.

    Purpose of the Study:

    • To develop and validate a micro-scale method for simultaneously determining T lymphocytes, B lymphocytes, and monocytes in mouse peripheral blood.
    • To provide a more efficient and reliable technique for immune cell analysis in murine models.

    Main Methods:

    • A micro method was devised requiring only 0.2 ml of whole mouse peripheral blood.
    • B lymphocytes and monocytes were determined simultaneously.
    • T lymphocytes were quantified using a cytotoxicity assay.

    Main Results:

    • The method successfully determined percentages of IgM and IgG bearing B lymphocytes.
    • Thy-1 bearing T lymphocytes and monocytes were accurately quantified.
    • The technique avoids cell population loss associated with flotation separation.

    Conclusions:

    • A novel, efficient micro method for assessing key immune cell populations in mouse blood has been established.
    • This method offers advantages over traditional techniques by minimizing sample volume and preventing cell loss.
    • The assay is suitable for analyzing immune cell profiles across different mouse strains.

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