Pan-cancer analysis of Sushi domain-containing protein 4 (SUSD4) and validated in colorectal cancer

Area of Science:

• Immunology
• Oncology
• Molecular Biology

Background:

• Sushi domain-containing protein 4 (SUSD4) is a complement regulatory protein involved in immune regulation.
• The specific role of SUSD4 in cancer progression has been largely unclear.
• This study investigates the association between SUSD4 and various cancer types.

Purpose of the Study:

• To determine the prognostic value of SUSD4 in diverse cancers.
• To explore the relationship between SUSD4 expression and the tumor microenvironment.
• To investigate the functional role of SUSD4 in colorectal cancer and its potential as a therapeutic target.

Main Methods:

• Bioinformatic analysis of SUSD4 expression across multiple cancer types.
• Correlation analysis with immune cell infiltration, immune genes, tumor heterogeneity, and cancer pathways.
• Functional validation in colorectal cancer cell lines, including SUSD4 knockdown.
• Drug sensitivity assessment using organoid models.

Main Results:

• SUSD4 expression levels are significantly associated with prognosis in multiple cancer types.
• SUSD4 expression correlates with immune cell infiltration, immune-related genes, tumor heterogeneity, and cancer pathways.
• Knockdown of SUSD4 inhibited colorectal cancer cell growth and affected the JAK/STAT pathway.
• SUSD4 expression showed a positive correlation with the IC50 of Selumetinib, YK-4-279, and Piperlongumine.

Conclusions:

• SUSD4 serves as a valuable prognostic biomarker for various cancers.
• SUSD4 influences cancer progression through immune modulation and pathway involvement.
• SUSD4 presents potential as a therapeutic target for cancer treatment.