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Updated: Jun 29, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
An aberration-free line scan confocal Raman imager and type classification and distribution detection of
Changwei Jiao1, Jiaqi Liao2, Sailing He3
1Centre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, China; Taizhou Hospital, Zhejiang University, Taizhou, China.
Abstract:
An aberration-free line scanning confocal Raman imager (named AFLSCRI) is developed to achieve rapid Raman imaging. As an application example, various types and sizes of MPs are identified through Raman imaging combined with a machine learning algorithm. The system has excellent performance with a spatial resolution of 2 µm and spectral resolution of 4 cm-1. Compared to traditional point-scanning Raman imaging systems, the detection speed is improved by 2 orders of magnitude. The pervasive nature of MPs results in their infiltration into the food chain, raising concerns for human health due to the potential for chemical leaching and the introduction of persistent organic pollutants. We conducted a series of experiments on various types and sizes of MPs. The system can give a classification accuracy of 98% for seven different types of plastics, and Raman imaging and species identification for MPs as small as 1 µm in diameter were achieved. We also identified toxic and harmful substances remaining in plastics, such as Dioctyl Phthalate (DOP) residues. This demonstrates a strong performance in microplastic species identification, size recognition and identification of hazardous substance contamination in microplastics.
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