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Updated: Jun 29, 2025
11:36
Glycan Node Analysis: A Bottom-up Approach to Glycomics
Published on: May 22, 2016
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Structural Elucidation and Prognostic Relevance of 297-11A-Sulfated Glycans in Ovarian Carcinoma
Daisuke Inoue1, Hitomi Hoshino2, Ya-Ying Chen3
1Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan.
Summary
New ovarian cancer biomarkers are crucial due to poor prognosis. This study identifies 297-11A-sulfated glycans, linked to MUC16, as potential predictors of ovarian carcinoma recurrence and shorter survival.
Area of Science:
- Glycobiology
- Oncology
- Biomarker Discovery
Background:
- Ovarian carcinoma often presents at advanced stages with poor prognosis, necessitating novel prognostic biomarkers.
- Previous studies indicated keratan sulfate (KS) expression in ovarian cancer, but detailed structures and significance were unclear.
Purpose of the Study:
- To characterize 297-11A-sulfated glycans in ovarian carcinoma using the 297-11A antibody.
- To determine the structural features and core protein association of these glycans.
- To evaluate the prognostic value of 297-11A-sulfated glycans in ovarian cancer patients.
Main Methods:
- Immunohistochemical analysis of 98 ovarian carcinoma cases with 297-11A antibody and glycosidases.
- Mass spectrometry analysis of OVCAR-3 cells to deduce glycan structure.
- Western blot analysis to assess association with MUC16 (CA125).
Main Results:
- 297-11A-sulfated glycans were primarily found in serous and endometrioid ovarian carcinomas.
- Structurally, these are O-glycans with partially sialylated, Gal-6-O-sulfated N-acetyllactosamine (LacNAc) on MUC16.
- Expression of these glycans correlated with shorter progression-free survival.
Conclusions:
- 297-11A-sulfated glycans are O-glycans associated with MUC16 in ovarian carcinoma.
- These glycans are differentially expressed across ovarian carcinoma subtypes.
- 297-11A-sulfated glycan expression serves as a potential predictor for ovarian carcinoma recurrence.
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