Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aspirin for cancer prevention in individuals with Lynch syndrome: first results from the CaPP3 multicentre, randomised, double-blind, non-inferiority trial.

The lancet. Gastroenterology & hepatology·2026
Same author

Functional characterization of the 9q34.13 locus identifies <i>RAPGEF1</i> as modulating risk for melanoma and nevi via RAS activation.

bioRxiv : the preprint server for biology·2026
Same author

Germline haploinsufficiency of MUTYH causes mutational signature SBS18 in multiple tumour types and specifically raises colorectal cancer risk.

NPJ precision oncology·2026
Same author

Implementation of surface-guided radiotherapy for motion management in liver SBRT: A scoping review and clinical workflow development.

Technical innovations & patient support in radiation oncology·2026
Same author

Sustained benefits of closed-loop transcranial alternating current stimulation (CL-tACS) on depression: a 12-week open-label clinical trial.

European archives of psychiatry and clinical neuroscience·2026
Same author

TGF-β Pathway-Based Polygenic Risk Score Modifies the Association between Red Meat Intake and Colorectal Cancer Risk: Application of a Novel Pathway-Based PRS Method.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026

Related Experiment Video

Updated: Jun 29, 2025

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from S&#233;zary Syndrome Patients for Transcriptomic Profiling
09:08

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

5.5K

Systemic Inflammation, the Peripheral Blood Transcriptome, and Primary Melanoma.

Juliette Randerson-Moor1, John Davies2, Mark Harland1

  • 1Division of Haematology and Immunology, Leeds Institute of Medical Research (LIMR), School of Medicine, University of Leeds, Leeds, United Kingdom.

The Journal of Investigative Dermatology
|April 7, 2024
PubMed
Summary

Systemic inflammation in melanoma patients is linked to poorer prognosis and reduced immune function, particularly in those with thicker tumors. Inflammatory markers like CRP and fibrinogen correlate with tumor thickness and mortality risk.

Keywords:
BreslowCD274PD-L1TILshsCRP

More Related Videos

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

424
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.4K

Related Experiment Videos

Last Updated: Jun 29, 2025

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from S&#233;zary Syndrome Patients for Transcriptomic Profiling
09:08

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

5.5K
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

424
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.4K

Area of Science:

  • Immunology
  • Oncology
  • Genomics

Background:

  • Systemic immunity and inflammation may impact melanoma prognosis.
  • Understanding these systemic factors is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the association between peripheral blood transcriptomes, systemic inflammation, and clinical outcomes in newly diagnosed melanoma patients.
  • To explore the relationship between inflammatory markers, tumor characteristics, and immune cell infiltration.

Main Methods:

  • Differential gene expression analysis of peripheral blood transcriptomes from 383 melanoma patients.
  • Correlation analysis of inflammatory markers (high-sensitivity CRP, fibrinogen) with tumor characteristics and gene expression.
  • Pathway analysis to identify dysregulated signaling in relation to tumor-infiltrating lymphocytes and inflammation.

Main Results:

  • Higher fibrinogen levels correlated with thicker primary tumors.
  • High-sensitivity CRP was associated with increased mortality and higher CD274 (PD-L1) expression, while CD274 expression was protective.
  • Transcriptomic analysis revealed downregulated immune cell signaling in patients with thicker tumors and STAT1 upregulation in those with brisk tumor-infiltrating lymphocytes.
  • Increased NF-kB signaling and reduced HLA class II expression were observed with higher inflammatory markers, suggesting impaired immune function.

Conclusions:

  • Peripheral blood transcriptomic data indicate reduced immune function in melanoma patients with thicker tumors and fewer tumor-infiltrating lymphocytes.
  • Systemic inflammatory markers are associated with thicker tumors and independently predict melanoma mortality.
  • Aberrant systemic inflammation appears to mediate reduced immune function in melanoma, highlighting its role in disease progression.