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Bioanalytical technologies using temperature-responsive polymers.

Kenichi Nagase1,2

  • 1Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. nagase@hiroshima-u.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|April 7, 2024
PubMed
Summary
This summary is machine-generated.

Temperature-responsive chromatography, using poly(N-isopropylacrylamide) (PNIPAAm), offers solvent-free separation for therapeutic drug monitoring. This technology shows great potential for clinical diagnosis and healthcare applications.

Keywords:
BiopharmaceuticalsBioseparationTemperature-responsive chromatographyTherapeutic drug monitoringThermoresponsive polymers

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Bioanalytical technologies are crucial for effective medical treatment and therapy.
  • Temperature-responsive chromatography (TRC) is a key bioanalytical technology utilizing poly(N-isopropylacrylamide) (PNIPAAm).
  • PNIPAAm's temperature-dependent hydrophobicity allows modulation of interactions without organic solvents.

Purpose of the Study:

  • To review recent advancements in TRC systems for therapeutic drug monitoring (TDM).
  • To summarize PNIPAAm-based separation methods for antibody drugs relevant to biopharmaceutical TDM.
  • To highlight the potential of TRC in clinical diagnosis and healthcare.

Main Methods:

  • Utilizing a poly(N-isopropylacrylamide) (PNIPAAm) modified stationary phase in chromatography columns.
  • Modulating hydrophobic interactions by adjusting column temperature.
  • Applying TRC for the separation and analysis of analytes, including antibody drugs.

Main Results:

  • TRC systems enable TDM without organic solvents, suitable for hospital settings.
  • PNIPAAm-based methods are effective for therapeutic drug monitoring of biopharmaceuticals.
  • TRC can simultaneously assess multiple medicines, aiding clinical diagnosis.

Conclusions:

  • Temperature-responsive chromatography is a promising, solvent-free technique for TDM.
  • This technology has significant potential for advancing medical treatment and healthcare.
  • TRC facilitates efficient analysis of biopharmaceuticals and simultaneous drug assessment.