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Updated: Jun 29, 2025
Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
Published on: November 25, 2011
Acellular carotid scaffold and evaluation the biological and biomechanical properties for tissue engineering
Farina Rashidi1, Mehdi Mohammadzadeh1, Arash Abdolmaleki2
1Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran.
Introduction:
The issues associated with the limitation of appropriate autologous vessels for vascular reconstruction via bypass surgery highlight the need for new alternative strategies based on tissue engineering. The present study aimed to prepare decellularized scaffolds from ovine carotid using chemical decellularization method.
Methods:
Ovine carotid were decellularized with Triton X-100 and tri-n-butyl phosphate (TnBP) at 37 °C. Histological analysis, biochemical tests, biomechanical assay and biocompatibility assay were used to investigate the efficacy of decellularization.
Results:
Decellularization method could successfully decellularize ovine carotid without leaving any cell remnants. Scaffolds showed minimal destruction of the three-dimensional structure and extracellular matrix, as well as adequate mechanical resistance and biocompatibility for cell growth and proliferation.
Conclusion:
Prepared acellular scaffold exhibited the necessary characteristics for clinical applications.
