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Effects of Rosa multiflora root extract on adipogenesis and lipogenesis in 3T3-L1 adipocytes and SD rat models
Kyoung Kon Kim1, Hye Rim Lee2, Sun Min Jang3
1Newgen Healthcare Co., Chuncheon 24232, Korea.
Background/Objectives:
Obesity is a major cause of metabolic disorders; to prevent obesity, research is ongoing to develop natural and safe ingredients with few adverse effects. In this study, we determined the anti-obesity effects of Rosa multiflora root extract (KWFD-H01) in 3T3-L1 adipocytes and Sprague-Dawley (SD) rats.
Materials/Methods:
The anti-obesity effects of KWFD-H01in 3T3-L1 adipocytes and SD rats were examined using various assays, including Oil Red O staining, gene expression analyses, protein expression analyses, and blood biochemical analyses.
Results:
KWFD-H01 reduced intracellular lipid accumulation and inhibited the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ), cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBPα), sterol regulatory element-binding transcription factor 1 (SREBP-1c), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) in 3T3-L1 cells. KWFD-H01 also reduced body weight, weight gain, and the levels of triglycerides, total and LDL-cholesterol, glucose, and leptin, while increasing high-density lipoprotein-cholesterol and adiponectin in SD rats. PPARγ, C/EBPα, SREBP-1c, ACC, and FAS protein expression was inhibited in the epididymal fat of SD rats.
Conclusion:
Overall, these results confirm the anti-obesity effects of KWFD-H01 in 3T3-L1 adipocytes and SD rats, indicating their potential as baseline data for developing functional health foods or pharmaceuticals to control obesity.
Insights
Rosa multiflora root extract (KWFD-H01) demonstrates significant anti-obesity effects by reducing fat accumulation and improving metabolic markers in cell and animal models. This natural extract shows promise for developing new obesity treatments.
Area of Science:
- Metabolic research
- Pharmacology
- Natural product chemistry
Background:
- Obesity is a primary driver of metabolic disorders, necessitating the development of safe, natural interventions.
- Research focuses on identifying natural compounds with anti-obesity properties and minimal adverse effects.
Purpose of the Study:
- To evaluate the anti-obesity potential of Rosa multiflora root extract (KWFD-H01).
- To investigate the effects of KWFD-H01 on adipocytes and in a rodent model of obesity.
Main Methods:
- In vitro assays using 3T3-L1 adipocytes, including Oil Red O staining and gene/protein expression analysis.
- In vivo studies in Sprague-Dawley (SD) rats involving body weight monitoring and blood biochemical analysis.
Main Results:
- KWFD-H01 inhibited lipid accumulation and key adipogenic gene/protein expression (PPARγ, C/EBPα, SREBP-1c, ACC, FAS) in 3T3-L1 cells.
- In SD rats, KWFD-H01 reduced body weight, weight gain, triglycerides, LDL-cholesterol, glucose, and leptin.
- KWFD-H01 increased HDL-cholesterol and adiponectin levels, while suppressing adipogenic markers in rat epididymal fat.
Conclusions:
- Rosa multiflora root extract (KWFD-H01) exhibits significant anti-obesity effects in both cellular and animal models.
- These findings support KWFD-H01 as a potential candidate for developing functional foods or pharmaceuticals to manage obesity.
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