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Updated: Jun 29, 2025

Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
YLR419W is the homolog of the mammalian translation initiation factor DHX29
Micheline Fromont-Racine1,2, Varun Khanna3, Alain Jacquier1,2
1Cytoplasmic mRNA surveillance in yeast, Institut Pasteur, Paris, Île-de-France, France.
Abstract:
27 years after the yeast genome sequencing, the function of many ORFs remain unknown. Despite the evolutionary distance between human and yeast, homology with the conserved DEAH/DExH-box helicase domains allowed us to list DHX29, DHX36 and DHX57 as three putative homologs of the yeast Ylr419wp. Functional studies first linked the Ylr419w protein to the translating ribosome and cross-linking and analysis of cDNA (CRAC) experiments determined the precise region of Ylr419wp in contact with the ribosome. It corresponds to the loop of the h16 helix in the 18S rRNA designing the translation initiation factor DHX29, as the functional homolog of Ylr419wp.
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