Related Experiment Video
Updated: Jun 29, 2025
Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
Helicobacter pylori causes gastric dysbacteriosis in chronic gastritis patients
Chao Cen1, Qiuying Du2, Bin Luo3
1Department of Gastroenterology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.
Abstract:
Gastric mucosal samples were procured and underwent the sequencing of 16S ribosomal RNA (16S rRNA) via Illumina high-throughput sequencing technology to explore the impact of Helicobacter pylori (H. pylori) infection on the composition of gastric flora in chronic gastritis (CG) patients. In the results, the operational taxonomic unit (OTU) analysis revealed an overlap of 5706 OTUs shared between the two groups. The top 5 abundance ranking (TOP5) phyla comprised Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Epsilonbacteraeota, while the TOP5 genus was Lachnospiraceae_NK4A136_group, Helicobacter, Bacteroides, Klebsiella, and Pseudomonas. In the metabolic pathways at the Kyoto Encyclopedia of Genes and Genomes (KEGG)_L3 level, conspicuous variations across seven functions were observed between the H. pylori-positive (HP_Pos) and H. pylori-negative (HP_Neg) groups. Subsequently, functional gene enrichment in KEGG pathways was further validated through animal experimentation. In contrast to the mice in the HP_Neg group, those infected with H. pylori manifested an infiltration of inflammatory cells, an augmentation in gastric acid secretion, and conspicuously elevated scores regarding gastric activity, along with heightened levels of malondialdehyde. In conclusion, CG patients infected with H. pylori displayed a disorder in gastric flora, furnishing a theoretical basis for the prophylaxis of H. pylori infection and its associated pathogenic ramifications.
Insights
Helicobacter pylori infection disrupts the gastric flora in chronic gastritis patients, leading to significant changes in microbial composition and function. This study provides a basis for preventing H. pylori infection and its harmful effects.
Area of Science:
- Microbiology
- Gastroenterology
- Genomics
Background:
- Chronic gastritis (CG) is often associated with Helicobacter pylori (H. pylori) infection.
- The impact of H. pylori on the gastric microbial community in CG patients requires further elucidation.
Purpose of the Study:
- To investigate the alterations in gastric flora composition and function in CG patients with H. pylori infection.
- To explore the pathogenic mechanisms of H. pylori-induced gastric dysbiosis.
Main Methods:
- 16S ribosomal RNA (16S rRNA) gene sequencing of gastric mucosal samples from H. pylori-positive (HP_Pos) and H. pylori-negative (HP_Neg) CG patients.
- Operational taxonomic unit (OTU) analysis and microbial community profiling.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for functional prediction.
- Animal experiments to validate functional gene enrichment and pathogenic effects.
Main Results:
- Significant differences in gastric flora composition were observed between HP_Pos and HP_Neg groups.
- Key phyla included Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Epsilonbacteraeota; key genera included Helicobacter, Lachnospiraceae_NK4A136_group, Bacteroides, Klebsiella, and Pseudomonas.
- Distinct variations in KEGG metabolic pathways were identified between the groups.
- H. pylori infection in mice led to inflammation, increased gastric acid secretion, elevated gastric activity, and higher malondialdehyde levels.
Conclusions:
- H. pylori infection induces gastric dysbiosis in CG patients.
- Altered gastric flora and function contribute to H. pylori-associated pathogenesis.
- Findings support the importance of H. pylori eradication for preventing gastric disease complications.
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