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Updated: Jun 29, 2025

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
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A high-throughput method for quantifying Drosophila fecundity.

Andreana Gomez1, Sergio Gonzalez2,3, Ashwini Oke4,5

  • 1University of California, San Francisco, Department of Anatomy.

Biorxiv : the Preprint Server for Biology
|April 8, 2024
PubMed
Summary

This study introduces a high-throughput Drosophila fecundity assay using automated fly transfer and imaging. The new method significantly improves efficiency and accuracy for studying aging, toxicology, and chemical exposure effects on egg laying.

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Area of Science:

  • * Utilizes Drosophila melanogaster as a model organism for broad biological research, including stem cell biology, nutrition, behavior, toxicology, and aging studies.
  • * Fecundity assays are crucial for longitudinal studies, assessing prolonged chemical exposure and aging effects due to their reliance on live subjects.

Background:

  • * Standard Drosophila fecundity assays are challenging for high-throughput screening due to the need for strict control over fly physiology and environmental conditions.
  • * Manual egg counting in response to diverse experimental conditions, such as dietary chemical additives, is labor-intensive and limits scalability.

Approach:

  • * Developed a novel multiwell fly culture system integrated with a 3D-printed fly transfer device for rapid and precise fly manipulation.
  • * Implemented RoboCam, a cost-effective robotic camera system, for automated image acquisition of multiwell plates.
  • * Created an automated image segmentation pipeline for accurate identification and quantification of Drosophila eggs.

Key Points:

  • * The automated system ensures robust and consistent egg laying throughout the experimental period.
  • * The image segmentation pipeline demonstrates high accuracy in egg quantification, with an R-squared value of 0.98 compared to ground truth.
  • * The method efficiently detects fecundity changes induced by dietary chemical exposure.

Conclusions:

  • * This integrated strategy significantly enhances the efficiency and reproducibility of high-throughput fecundity assays.
  • * The automated approach facilitates large-scale screening of various media conditions and their impact on Drosophila reproduction.
  • * Enables more comprehensive investigations into factors affecting fecundity, from aging to chemical interventions.