Related Experiment Video
Updated: Jun 29, 2025

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
Published on: March 24, 2019
Protein crotonylation: Basic research and clinical diseases
Dongling Li1,2, Ling Lin2, Fan Xu1,2
1School of Medicine, Chongqing University, Chongqing, 400044, China.
Crotonylation, a distinct post-translational modification, impacts gene expression and protein function. Understanding its roles in histones and non-histones offers potential therapeutic targets for diseases.
Area of Science:
- Biochemistry
- Molecular Biology
- Epigenetics
Background:
- Crotonylation is a conserved post-translational modification distinct from acetylation.
- It modifies both histone and non-histone proteins, influencing cellular processes.
Purpose of the Study:
- To review and summarize the regulatory mechanisms of histone and non-histone crotonylation.
- To explore crotonylation sites as potential therapeutic targets for disease treatment.
Main Methods:
- Literature review of existing studies on crotonylation.
- Analysis of crotonylation's role in gene expression and protein function.
- Investigation of crotonylation's involvement in disease pathogenesis.
Main Results:
- Histone crotonylation primarily regulates gene expression via transcriptional control.
- Non-histone crotonylation affects protein activity, localization, stability, and interactions.
- Altered protein expression and function due to crotonylation impact cellular physiology and can lead to disease.
Conclusions:
- Crotonylation plays a critical role in cellular functions and disease development.
- Targeting specific crotonylation sites presents a promising strategy for precise disease intervention.
More Related Videos
11:49Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
Published on: August 28, 2021
09:29Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Related Concept Videos
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Smooth Endoplasmic Reticulum
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...