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Updated: Jun 29, 2025

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Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer
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Carotenoids and Tocopherol Profiling in Fleshy Fruits
Bruno Silvestre Lira1, Aline Bertinatto Cruz1, Magdalena Rossi1
1Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|April 8, 2024
Abstract:
Carotenoids and tocopherols are among the most powerful lipophilic antioxidants accumulated in fruit and vegetable crops. This chapter describes a method for the separation and quantification of carotenoids/chlorophylls and tocopherols based on microextraction followed by reverse- and normal-phase HPLC, respectively. Using this method, high-throughput, accurate analysis of these compounds can be performed in leaf and fruit samples.

