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An Enzyme Assay Kit for GABA Quantification in Plant Tissues
Tatsuya Nishiyama1, Noriko Wada1, Hitoshi Kusakabe2
1Life Science Research Centre, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
Abstract:
Gamma-aminobutyric acid (GABA) is an amino acid that has a role as a signaling molecule. In plants, its involvement in stress responses is widely investigated. A newly developed method of quantification of GABA is described in this chapter. The assay kit consisting of three bacterial enzymes enables easy but accurate measurement of GABA (~200 mg/mL) based on the serial enzymatic reaction leading to dye formation. The method was successfully applied to measure the GABA content in several plant tissues.
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